A fast, Simple and accurate liquid chromatographic (HPLC) and thin layer chromatographic (HPTLC-densitometry) methods for determination of camylofin dihydrochloride (CAM) in tablets were elaborated. The first method was based on isocratic reversed phase liquid chromatography by using RP ODS-C18 column with a mobile phase consisting of acetonitrile: 25mM potassium dihydrogen phosphate (70:30, v/v) adjusted to pH 7 with triethylamine at a flow rate of 1.2 mL/min and UV detection at 215 nm. The calibration curve of camylofin was linear in the range of 50-300 µg.mL-1 with correlation of coefficient r >0.999. The second thin layer chromatographic method employed by using pre-coated silica gel G60–F254 aluminum sheet using mobile phase chloroform:methanol:ammonia (6:4:0.1,v/v) and quantitation was achieved using spectrodensitometrically at 215 nm. The calibration curve of camylofin was linear in the range of 1500-9000 ng.spot-1 with correlation of coefficient r>0.999. The validity of the methods was confirmed using the recovery studies, precision specificity and limit of detection. Both techniques were applied successfully for the analysis of camylofin in bulk drug and pharmaceutical dosage form. The results obtained from both procedures were statistically compared using the Student’s-t and F-variance ratio tests.
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